Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens

dc.contributor.authorIqbal, Muhammad Arsalan
dc.contributor.authorAli, Asghar
dc.contributor.authorHadlich, Frieder
dc.contributor.authorOster, Michael
dc.contributor.authorReyer, Henry
dc.contributor.authorTrakooljul, Nares
dc.contributor.authorSommerfeld, Vera
dc.contributor.authorRodehutscord, Markus
dc.contributor.authorWimmers, Klaus
dc.contributor.authorPonsuksili, Siriluck
dc.date.accessioned2024-09-03T08:32:16Z
dc.date.available2024-09-03T08:32:16Z
dc.date.issued2021de
dc.description.abstractPhosphorus (P) and calcium (Ca) are critical for egg production in laying hens. Most of P in plant-based poultry diet is bound as phytic acid and needs to be hydrolysed before absorption. To increase P bioavailability, exogenous phytases or bioavailable rock phosphate is added in feed. There is growing evidence of the importance of miRNAs as the epicentre of intestinal homeostasis and functional properties. Therefore, we demonstrated the expression of miRNA profiles and the corresponding target genes due to the different levels of P (recommended vs. 20% reduction) and/or Ca (recommended vs. 15% reduction) in feed. Jejunal miRNA profiles of Lohmann Selected Leghorn (LSL) and Lohmann Brown (LB) laying hens strains were used (nā€‰=ā€‰80). A total of 34 and 76 miRNAs were differentially expressed (DE) in the different diet groups within LSL and LB strains respectively. In LSL, the DE miRNAs and their targets were involved in calcium signaling pathway, inositol phosphate metabolism, and mitochondrial dysfunction. Similarly, in LB miRNAs targets were enriched in metabolic pathways such as glutathione metabolism, phosphonate metabolism and vitamin B6 metabolism. Our results suggest that both strains employ different intrinsic strategies to cope with modulated P and Ca supply and maintain mineral homeostasis.en
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16387
dc.identifier.urihttps://doi.org/10.1038/s41598-021-92932-3
dc.language.isoengde
dc.rights.licensecc_byde
dc.source2045-2322de
dc.sourceScientific Reports; Vol. 11, No. 1 (2021) 13534de
dc.subjectBiotechnology
dc.subjectGastroenterology
dc.subjectGenetics
dc.subject.ddc630
dc.titleDietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hensen
dc.type.diniArticle
dcterms.bibliographicCitationScientific reports, 11 (2021), 1, 13534. https://doi.org/10.1038/s41598-021-92932-3. ISSN: 2045-2322
dcterms.bibliographicCitation.issn2045-2322
dcterms.bibliographicCitation.issue1
dcterms.bibliographicCitation.journaltitleScientific reports
dcterms.bibliographicCitation.volume11
local.export.bibtex@article{Iqbal2021, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16387}, doi = {10.1038/s41598-021-92932-3}, author = {Iqbal, Muhammad Arsalan and Ali, Asghar and Hadlich, Frieder et al.}, title = {Dietary phosphorus and calcium in feed affects miRNA profiles and their mRNA targets in jejunum of two strains of laying hens}, journal = {Scientific reports}, year = {2021}, volume = {11}, number = {1}, }
local.export.bibtexAuthorIqbal, Muhammad Arsalan and Ali, Asghar and Hadlich, Frieder et al.
local.export.bibtexKeyIqbal2021
local.export.bibtexType@article

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