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Article
2024

Arthropods as vectors of grapevine trunk disease pathogens: Quantification of Phaeomoniella chlamydospora on arthropods and mycobiome analysis of earwig exoskeletons

Abstract (English)

Viticulture worldwide is challenged by grapevine trunk diseases (GTDs). Involvement of arthropods in the dissemination process of GTD pathogens, notably esca pathogens, is indicated after detection of associated pathogens on arthropod exoskeletons, and demonstration of transmission under artificial conditions. The present study is the first to quantify spore loads via qPCR of the esca-relevant pathogen Phaeomoniella chlamydospora on arthropods collected in German vineyards, i.e., European earwigs (Forficula auricularia), ants (Formicidae), and two species of jumping spiders (Marpissa muscosa and Synageles venator). Quantification of spore loads showed acquisition on exoskeletons, but most arthropods carried only low amounts. The mycobiome on earwig exoskeletons was described for the first time to reveal involvement of earwigs in the dispersal of GTDs in general. Metabarcoding data support the potential risk of earwigs as vectors for predominantly Pa. chlamydospora and possibly Eutypa lata (causative agent of Eutypa dieback), as respective operational taxonomical unit (OTU) assigned genera had relative abundances of 6.6% and 2.8% in total reads, even though with great variation between samples. Seven further GTD-related genera were present at a very low level. As various factors influence the successful transmission of GTD pathogens, we hypothesize that arthropods might irregularly act as direct vectors. Our results highlight the importance of minimizing and protecting pruning wounds in the field.

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Journal of Fungi, 10 (2024), 4. https://doi.org/10.3390/jof10040237. ISSN: 2309-608X
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630 Agriculture

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@article{Brandenburg2024, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16100}, doi = {10.3390/jof10040237}, author = {Brandenburg, Elisa Maria and Voegele, Ralf Thomas and Fischer, Michael et al.}, title = {Arthropods as vectors of grapevine trunk disease pathogens: Quantification of Phaeomoniella chlamydospora on arthropods and mycobiome analysis of earwig exoskeletons}, journal = {Journal of Fungi}, year = {2024}, volume = {10}, number = {4}, }