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Article
2021
Bacillus subtilis high cell density fermentation using a sporulation-deficient strain for the production of surfactin
Bacillus subtilis high cell density fermentation using a sporulation-deficient strain for the production of surfactin
Abstract (English)
Bacillus subtilis 3NA is a strain capable of reaching high cell densities. A surfactin producing sfp+ variant of this strain, named JABs32, was utilized in fed-batch cultivation processes. Both a glucose and an ammonia solution were fed to set a steady growth rate μ of 0.1 h-1. In this process, a cell dry weight of up to 88 g L-1 was reached after 38 h of cultivation, and surfactin titers of up to 26.5 g L-1 were detected in this high cell density fermentation process, achieving a YP/X value of 0.23 g g-1 as well as a qP/X of 0.007 g g-1 h-1. In sum, a 21-fold increase in surfactin titer was obtained compared with cultivations in shake flasks. In contrast to fed-batch operations using Bacillus subtilis JABs24, an sfp+ variant derived from B. subtilis 168, JABs32, reached an up to fourfold increase in surfactin titers using the same fed-batch protocol. Additionally, a two-stage feed process was established utilizing strain JABs32. Using an optimized mineral salt medium in this high cell density fermentation approach, after 31 h of cultivation, surfactin titers of 23.7 g L-1 were reached with a biomass concentration of 41.3 g L-1, thus achieving an enhanced YP/X value of 0.57 g g-1 as well as a qP/X of 0.018 g g-1 h-1. The mutation of spo0A locus and an elongation of AbrB in the strain utilized in combination with a high cell density fed-batch process represents a promising new route for future enhancements on surfactin production.
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Applied microbiology and biotechnology, 105 (2021), 10, 4141-4151.
https://doi.org/10.1007/s00253-021-11330-x.
ISSN: 1432-0614
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English
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660 Chemical engineerin
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@article{Klausmann2021,
url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16438},
doi = {10.1007/s00253-021-11330-x},
author = {Klausmann, Peter and Hennemann, Katja and Hoffmann, Mareen et al.},
title = {Bacillus subtilis High Cell Density Fermentation Using a Sporulation-Deficient Strain for the Production of Surfactin},
journal = {Applied microbiology and biotechnology},
year = {2021},
volume = {105},
number = {10},
}