A high‐confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution

Simple item page
dc.contributor.authorGombos, Sven
dc.contributor.authorMiras, Manuel
dc.contributor.authorHowe, Vicky
dc.contributor.authorXi, Lin
dc.contributor.authorPottier, Mathieu
dc.contributor.authorKazemein Jasemi, Neda S.
dc.contributor.authorSchladt, Moritz
dc.contributor.authorEjike, J. Obinna
dc.contributor.authorNeumann, Ulla
dc.contributor.authorHänsch, Sebastian
dc.contributor.authorKuttig, Franziska
dc.contributor.authorZhang, Zhaoxia
dc.contributor.authorDickmanns, Marcel
dc.contributor.authorXu, Peng
dc.contributor.authorStefan, Thorsten
dc.contributor.authorBaumeister, Wolfgang
dc.contributor.authorFrommer, Wolf B.
dc.contributor.authorSimon, Rüdiger
dc.contributor.authorSchulze, Waltraud X.
dc.date.accessioned2024-09-03T08:32:10Z
dc.date.available2024-09-03T08:32:10Z
dc.date.issued2023de
dc.description.abstractPlasmodesmata (PD) facilitate movement of molecules between plant cells. Regulation of this movement is still not understood. Plasmodesmata are hard to study, being deeply embedded within cell walls and incorporating several membrane types. Thus, structure and protein composition of PD remain enigmatic. Previous studies of PD protein composition identified protein lists with few validations, making functional conclusions difficult. We developed a PD scoring approach in iteration with large‐scale systematic localization, defining a high‐confidence PD proteome of Physcomitrium patens (HC300). HC300, together with bona fide PD proteins from literature, were placed in Pddb. About 65% of proteins in HC300 were not previously PD‐localized. Callose‐degrading glycolyl hydrolase family 17 (GHL17) is an abundant protein family with representatives across evolutionary scale. Among GHL17s, we exclusively found members of one phylogenetic clade with PD localization and orthologs occur only in species with developed PD. Phylogenetic comparison was expanded to xyloglucan endotransglucosylases/hydrolases and Exordium‐like proteins, which also diversified into PD‐localized and non‐PD‐localized members on distinct phylogenetic clades. Our high‐confidence PD proteome HC300 provides insights into diversification of large protein families. Iterative and systematic large‐scale localization across plant species strengthens the reliability of HC300 as basis for exploring structure, function, and evolution of this important organelle.en
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/16365
dc.identifier.urihttps://doi.org/10.1111/nph.18730
dc.language.isoengde
dc.rights.licensecc_by-ncde
dc.source1469-8137de
dc.sourceNew Phytologist; Vol. 238, No. 2 (2023), 637-653de
dc.subjectCell wall‐modifying enzymesen
dc.subjectCell‐to‐cell communicationen
dc.subjectEvolution of multicellularityen
dc.subjectPlasmodesmata proteomeen
dc.subjectPhyscomitrium patensen
dc.subject.ddc570
dc.titleA high‐confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolutionen
dc.type.diniArticle
dcterms.bibliographicCitationNew phytologist, 238 (2023), 2, 637-653. https://doi.org/10.1111/nph.18730. ISSN: 1469-8137
dcterms.bibliographicCitation.issn1469-8137
dcterms.bibliographicCitation.issue2
dcterms.bibliographicCitation.journaltitleNew phytologist
dcterms.bibliographicCitation.volume238
local.export.bibtex@article{Gombos2023, url = {https://hohpublica.uni-hohenheim.de/handle/123456789/16365}, doi = {10.1111/nph.18730}, author = {Gombos, Sven and Miras, Manuel and Howe, Vicky et al.}, title = {A high‐confidence Physcomitrium patens plasmodesmata proteome by iterative scoring and validation reveals diversification of cell wall proteins during evolution}, journal = {New phytologist}, year = {2023}, volume = {238}, number = {2}, }
local.export.bibtexAuthorGombos, Sven and Miras, Manuel and Howe, Vicky et al.
local.export.bibtexKeyGombos2023
local.export.bibtexType@article

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
NPH_NPH18730.pdf
Size:
5.84 MB
Format:
Adobe Portable Document Format
Download
No Thumbnail Available
Name:
nph18730-sup.zip
Size:
2.21 MB
Format:
Unknown data format
Download

Collections

Institut für Biologie