Tannic acid and ethacridine lactate attenuate markers of stress-induced intestinal barrier dysfunctions in murine small intestinal organoids

dc.contributor.authorFilipe Rosa, Louisa
dc.contributor.authorGonda, Steffen
dc.contributor.authorRoese, Nadine
dc.contributor.authorBischoff, Stephan C.
dc.contributor.corporateFilipe Rosa, Louisa; Institute of Nutritional Medicine, University of Hohenheim, Fruwirthstr. 12, 70599 Stuttgart, Germany;
dc.contributor.corporateGonda, Steffen; MEDICE Arzneimittel Pütter GmbH & Co. KG, Kuhloweg 37, 58638 Iserlohn, Germany
dc.contributor.corporateRoese, Nadine; MEDICE Arzneimittel Pütter GmbH & Co. KG, Kuhloweg 37, 58638 Iserlohn, Germany
dc.contributor.corporateBischoff, Stephan C.; Institute of Nutritional Medicine, University of Hohenheim, Fruwirthstr. 12, 70599 Stuttgart, Germany;
dc.contributor.editorKwok, Hang Fai (Henry)
dc.contributor.editorAlaimo, Alessandro
dc.date.accessioned2025-07-03T13:05:44Z
dc.date.available2025-07-03T13:05:44Z
dc.date.issued2025
dc.date.updated2025-05-13T11:18:53Z
dc.description.abstract(1) Background: Tannacomp® is a drug consisting of tannin albuminate, a complex of tannic acid (TA) and ethacridine lactate (Eta) used for treating acute and traveler’s diarrhea. TA is thought to modulate gastrointestinal barrier function, but the underlying mechanisms and whether Eta has similar effects remains unclear. (2) Methods: to investigate the effects of TA and Eta on the intestinal barrier, stress responses were induced in murine intestinal organoids by lipopolysaccharide (LPS) exposure or withdrawal of growth factors from cell culture medium (GFRed). Further, organoids were exposed to either TA (0.01 mg/mL) or Eta (0.002 mg/mL) and markers of inflammatory response and gut barrier function were assessed. (3) Results: TA and Eta reduced several inflammatory markers such as interleukin 6, interleukin 1β, tumor necrosis factor α, and myeloid differentiation primary response 88 in stressed organoids. In addition, TA and Eta attenuated LPS- and GFRed-mediated gut barrier dysfunctions, with normalization of tight junction, adherent junction and mucin gene expression and reduction of Nod2- and matrix metalloproteinase 7-dependent activation of antimicrobial peptides. (4) Conclusions: our data show that TA and Eta modulate markers of inflammation and the intestinal barrier and suggest novel mechanisms of action of this drug that could broaden its treatment indications.
dc.description.sponsorshipThis research was funded by MEDICE Arzneimittel Pütter GmbH & Co KG.
dc.description.sponsorshipMEDICE Arzneimittel Pütter GmbH & Co KG
dc.identifier.urihttps://doi.org/10.3390/biom15050650
dc.identifier.urihttps://hohpublica.uni-hohenheim.de/handle/123456789/17664
dc.language.isoeng
dc.rights.licensecc_by
dc.subjectTannic acid
dc.subjectEthacridine lactate
dc.subjectGastrointestinal barrier
dc.subjectTight junctions
dc.subjectAntimicrobial peptides
dc.subjectDiarrhea
dc.subjectGastroenteritis
dc.subjectMucosal protection
dc.subjectInflammation
dc.subjectOrganoids
dc.subject.ddc610
dc.titleTannic acid and ethacridine lactate attenuate markers of stress-induced intestinal barrier dysfunctions in murine small intestinal organoidsen
dc.type.diniArticle
dcterms.bibliographicCitationBiomolecules, 15 (2025), 5, 650. https://doi.org/10.3390/biom15050650. ISSN: 2218-273X
dcterms.bibliographicCitation.articlenumber650
dcterms.bibliographicCitation.issn2218-273X
dcterms.bibliographicCitation.issue5
dcterms.bibliographicCitation.journaltitleBiomolecules
dcterms.bibliographicCitation.originalpublishernameMDPI
dcterms.bibliographicCitation.originalpublisherplaceBasel
dcterms.bibliographicCitation.volume15
local.export.bibtex@article{Filipe Rosa2025, doi = {10.3390/biom15050650}, author = {Filipe Rosa, Louisa and Gonda, Steffen and Roese, Nadine et al.}, title = {Tannic Acid and Ethacridine Lactate Attenuate Markers of Stress-Induced Intestinal Barrier Dysfunctions in Murine Small Intestinal Organoids}, journal = {Biomolecules}, year = {2025}, volume = {15}, number = {5}, }
local.export.bibtexAuthorFilipe Rosa, Louisa and Gonda, Steffen and Roese, Nadine et al.
local.export.bibtexKeyFilipe Rosa2025
local.export.bibtexType@article
local.title.fullTannic Acid and Ethacridine Lactate Attenuate Markers of Stress-Induced Intestinal Barrier Dysfunctions in Murine Small Intestinal Organoids

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