Browsing by Subject "Stem rust resistance"

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Mapping and validating stem rust resistance genes directly in self-incompatible genetic resources of winter rye
(2021) Gruner, Paul; Schmitt, Anne-Kristin; Flath, Kerstin; Piepho, Hans-Peter; Miedaner, Thomas
Key message: Individual stem rust resistance genes could be directly mapped within self-incompatible rye populations. Abstract: Genetic resources of rye (Secale cereale L.) are cross-pollinating populations that can be highly diverse and are naturally segregating. In this study, we show that this segregation could be used for mapping stem rust resistance. Populations of pre-selected donors from the Russian Federation, the USA and Austria were tested on a single-plant basis for stem rust resistance by a leaf-segment test with three rust isolates. Seventy-four plants per population were genotyped with a 10 K-SNP chip. Using cumulative logit models, significant associations between the ordinal infection score and the marker alleles could be found. Three different loci (Pgs1, Pgs2, Pgs3) in three populations were highly significant, and resistance-linked markers could be validated with field experiments of an independent seed sample from the original population and were used to fix two populations for resistance. We showed that it is possible to map monogenically inherited seedling resistance genes directly in genetic resources, thus providing a competitive alternative to linkage mapping approaches that require a tedious and time-consuming inbreeding over several generations.