Repository logo
Log In
Log in as University member:
Log in as external user:
Have you forgotten your password?

Please contact the hohPublica team if you do not have a valid Hohenheim user account (hohPublica@uni-hohenheim.de)
Hilfe
  • English
  • Deutsch
    Communities & Collections
    All of hohPublica
Log In
Log in as University member:
Log in as external user:
Have you forgotten your password?

Please contact the hohPublica team if you do not have a valid Hohenheim user account (hohPublica@uni-hohenheim.de)
Hilfe
  • English
  • Deutsch
  1. Home
  2. Browse by Subject

Browsing by Subject "Fluorescent conjugates"

Type the first few letters and click on the Browse button
Now showing 1 - 1 of 1
  • Results Per Page
  • Sort Options
  • Loading...
    Thumbnail Image
    Publication
    Concerning the photophysics of fluorophores towards tailored bioimaging compounds: A case study involving S100A9 inflammation markers
    (2023) Steiner, Simon T.; Maisuls, Iván; Junker, Anna; Fritz, Günter; Faust, Andreas; Strassert, Cristian A.; Steiner, Simon T.; European Institute for Molecular Imaging, Westfälische Wilhelms-Universität Münster, Münster, Germany; Maisuls, Iván; Center for Nanotechnology, Westfälische Wilhelms-Universität Münster, Münster, Germany; Junker, Anna; European Institute for Molecular Imaging, Westfälische Wilhelms-Universität Münster, Münster, Germany; Fritz, Günter; Cellular Microbiology, Institute of Biology, University of Hohenheim, Stuttgart, Germany; Faust, Andreas; European Institute for Molecular Imaging, Westfälische Wilhelms-Universität Münster, Münster, Germany; Strassert, Cristian A.; Center for Nanotechnology, Westfälische Wilhelms-Universität Münster, Münster, Germany
    A full understanding concerning the photophysical properties of a fluorescent label is crucial for a reliable and predictable performance in biolabelling applications. This holds true not only for the choice of a fluorophore in general, but also for the correct interpretation of data, considering the complexity of biological environments. In the frame of a case study involving inflammation imaging, we report the photophysical characterization of four fluorescent S100A9-targeting compounds in terms of UV–vis absorption and photoluminescence spectroscopy, fluorescence quantum yields (ΦF) and excited state lifetimes (τ) as well as the evaluation of the radiative and non-radiative rate constants (kr and knr, respectively). The probes were synthesized based on a 2-amino benzimidazole-based lead structure in combination with commercially available dyes, covering a broad color range from green (6-FAM) over orange (BODIPY-TMR) to red (BODIPY-TR) and near-infrared (Cy5.5) emission. The effect of conjugation with the targeting structure was addressed by comparison of the probes with their corresponding dye-azide precursors. Additionally, the 6-FAM and Cy5.5 probes were measured in the presence of murine S100A9 to determine whether protein binding influences their photophysical properties. An interesting rise in ΦF upon binding of 6-FAM-SST177 to murine S100A9 enabled the determination of its dissociation equilibrium constant, reaching up to KD = 324 nM. This result gives an outlook for potential applications of our compounds in S100A9 inflammation imaging and fluorescence assay developments. With respect to the other dyes, this study demonstrates how diverse microenvironmental factors can severely impair their performance while rendering them poor performers in biological media, showing that a preliminary photophysical screening is key to assess the suitability of a particular luminophore.

  • Contact
  • FAQ
  • Cookie settings
  • Imprint/Privacy policy