Browsing by Subject "Enzyme addition assay"

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    Release of glucose from dissolved and mineral‐bound organic matter by enzymatic hydrolysis
    (2023) Lenhardt, Katharina R.; Brandt, Luise; Poll, Christian; Rennert, Thilo; Kandeler, Ellen
    Sorption of dissolved organic matter (DOM) by poorly crystalline minerals during their formation may protect large amounts of carbon in soils from mineralization. We investigated the bioavailability of carbohydrates in DOM and after co-precipitation with short-range ordered aluminosilicates. Carbohydrates originated from soil solutions collected in situ at two depths of a Dystric Cambisol, and from litter extracts. Quantification of substrate-specific degradability was achieved by the addition of β-glucosidase at an optimal concentration and subsequent determination of glucose release. Depending on DOM composition, 0.6–41.4 mg g−1 C−1 of glucose was enzymatically released from dissolved carbohydrates. Co-precipitated carbohydrates were partially accessible, resulting in a glucose release of 0.7–5.2 mg g−1 C−1. Restricted enzymatic depolymerization due to co-precipitation may contribute to accumulation of easily degradable substrates in soils.