Browsing by Subject "Electron transport"

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Data on transgenerational memory effects of photosynthetic efficiency of twelve wheat varieties under elevated carbon dioxide concentration and reduced soil water availability
(2025) Berauer, Bernd J.; Chaudhary, Suraj; Kottmann, Lorenz; Schweiger, Andreas H.
This data represents ACi curves of twelve winter wheat varieties, which were grown under elevated and ambient CO2 concentrations within a FACE experiment and the subsequent F1 generation was exposed to ambient and elevated CO2 concentrations in a highly controlled environment using climate chambers. The 12 winter wheat genotypes (Triticum aestivum L.) were selected based on their susceptibilty to leaf rust (Puccinia triticina Eriks.) and Fusarium head blight (Fusarium graminearum Schwabe) according to the descriptive variety list of the German Federal Office of Plant Varietes (Beschreibende Sortenliste, Bundessortenamt 2024). The aim was to obtain a diverse set of varieties with the widest possible range of susceptibilities to leaf rust and fusarium head blight. Photosynthesis was measured using the novel Dynamic Assimilation Technique, thus not with the common steady-state approach. The individual wheat plants were measured twice, once under saturating soil water availability (θFC) and once under reduced soil water availability (θcsoil). θcsoil represents the gravimetric water content when the soil matric potential drops below the root matric potential, thus the onset of plant drought stress (sensu Cai et al. [2]). The photosynthesis data was used to fit ACi curves and extract the maximum Rubisco carboxylation rate [Vcmax], maximum rate of electron transport [Jmax] and dark respiration [Rd]. At both measurements we determined BBCH and plant height to quantify plant morphological development, as well as leaf water potential to quantify plant ecohydrologic status. At the end of the experiment, biomass was harvested and reported. Further, we provide environmental data of the climate chambers in use. Within the data repository, we provide comprehensive experimental data on the investigation of transgenerational memory effects on photosynthetic efficiency. We provide photosynthetic raw data as well as processed (merged) and derived (extracted ACi fit) data. Additionally, we provide the R-code to reproduce the calculation of the derived parameters. Data on transgenerational memory effects (that is, the influence of the parental environment on offspring phenotype and performance) are scarce, i.e. on the adaptive capacity of the photosynthetic apparatus. Thus, the data provided here can contribute to closing this gap. The highly controlled environment allows to closely investigate cause-effect relationships, thereby contributing to a mechanistic understanding of the transgenerational memory effects on photosynthetic efficiency and how this is altered by reduced soil water availability. By using a recently developed methodological approach, the data contributes to further investigate the quality of the method and establish it within the field of plant ecophysiology.
High-throughput field phenotyping reveals genetic variation in photosynthetic traits in durum wheat under drought
(2021) Zendonadi dos Santos, Nícolas; Piepho, Hans‐Peter; Condorelli, Giuseppe Emanuele; Licieri Groli, Eder; Newcomb, Maria; Ward, Richard; Tuberosa, Roberto; Maccaferri, Marco; Fiorani, Fabio; Rascher, Uwe; Muller, Onno
Chlorophyll fluorescence (ChlF) is a powerful non‐invasive technique for probing photosynthesis. Although proposed as a method for drought tolerance screening, ChlF has not yet been fully adopted in physiological breeding, mainly due to limitations in high‐throughput field phenotyping capabilities. The light‐induced fluorescence transient (LIFT) sensor has recently been shown to reliably provide active ChlF data for rapid and remote characterisation of plant photosynthetic performance. We used the LIFT sensor to quantify photosynthesis traits across time in a large panel of durum wheat genotypes subjected to a progressive drought in replicated field trials over two growing seasons. The photosynthetic performance was measured at the canopy level by means of the operating efficiency of Photosystem II (Fq′/Fm′) and the kinetics of electron transport measured by reoxidation rates (Fr1′ and Fr2′). Short‐ and long‐term changes in ChlF traits were found in response to soil water availability and due to interactions with weather fluctuations. In mild drought, Fq′/Fm′ and Fr2′ were little affected, while Fr1′ was consistently accelerated in water‐limited compared to well‐watered plants, increasingly so with rising vapour pressure deficit. This high‐throughput approach allowed assessment of the native genetic diversity in ChlF traits while considering the diurnal dynamics of photosynthesis.