Browsing by Subject "Docosahexaenoic acid"

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    Das Potenzial von Falschem Mehltau als Quelle von Omega-3-Fettsäuren für die menschliche Ernährung
    (2009) Anderle, Ann-Marie; Spring, Otmar
    The absolute EPA ((5Z, 8Z, 11Z, 14Z, 17Z)-eicosapentaenoic acid) contents of the downy mildews of sunflowers (Plasmopara halstedii) and lettuce (Bremia lactucae) were quantified by means of GC-FID. The EPA content in sporangia of Bremia lactucae varied between 8 to 13 mg per dry weight. As at the institute of botany a collection of genetically identical sporangia strains of Plasmopara halstedii was established, the natural variation of EPA in four genetically different sporangia strains of Plasmopara halstedii on 10 different sunflower cultivars or -lines (15 days old seedlings) was investigated. The variance in EPA between the sporangia strains (LS-13.12.05-C6, BL-11.06.02-A4z, GG-16.10.97-A25, HE-10.01.06-A8) was only low (18-25 mg EPA per g dry weight). In contrast, the specific variation of EPA in infected sunflower seedlings (Giganteus, HA 821, HA 304, RHA 265, RHA 274, PM 13, 799-2, PM 17, 803-1, DM-2) was relatively high (0,28 to 1,10 mg EPA per g dry weight). Additionally three stages (minimum, optimum, maximum) of nitrogen fertilization were tested for their influence on the EPA content in infected sunflower seedlings. Statistical analysis was carried out by the program SAS. Analysis of variance based on F-tests and multiple t-tests. The influence of hydroponic fertilization (0.1 mM, 1 mM, 5 mM) on EPA in infected sunflower seedlings was high. The nitrogen dose of 5 mM almost doubled the EPA content in infected sunflower seedlings from 1 to almost 2 mg EPA per g dry weight. However, this content is not enough (by factor 10) to serve directly for human nutrition. Therefore at last a food chain trial with Bremia lactucae- infected lettuce was carried out in a cooperation project by several institutes of the Universtiy of Hohenheim. Infected lettuce was fed to hens. The omega-3-fatty acid content per egg was almost doubled (80 to 136 mg) if 10% lettuce was given to hen´s food. The infection with EPA containing downy mildew showed no effects.