Browsing by Person "Winterfeld, Sophie"
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Publication In vitro Interaktionsstudie der bakteriellen Insertase YidC von Escherichia coli mit seinem Substrat Pf3 coat Protein durch Fluoreszenzspektroskopie(2011) Winterfeld, Sophie; Kuhn, AndreasWith fluorescence spectroscopy the insertion of Pf3 coat protein into YidC proteoliposomes could be characterized. Therefore the binding could be determined by fluorescence titration and with help of single-molecule spectroscopy the insertion process of Pf3 coat protein was analyzed with FRET. Thus the distances of the proteins were determined and the time dependent insertion was observed.