Landesanstalten

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A full-scale study on efficiency and emissions of an agricultural biogas plant
(2013) Nägele, Hans-Joachim; Jungbluth, Thomas
In this study we focused on process engineering for the conversion of biomass, and utilization of the gas obtained by fermentation. Several topics regarding efficiency and emissions have been addressed by conducting intensive and long-term measurements. In detail, our objectives were (1) to conduct long-term measurements of the electric energy consumption of the biogas plant and its individual components and examination of energy-saving potentials; (2) to develop a method to measure mixing quality in the digester and to examine the mixing quality by measuring nutrient distribution in the digester with different agitator setups; (3) measure the influence of maintenance strategies on efficiency and emissions at long-term operation in practical application; (4) examine the efficiency of an external biological desulfurization plant under practical conditions to enhance biogas fuel quality. The results of electric energy measurement over a period of two years showed that a percentage of 8.5% (in 2010) and 8.7% (in 2011) of the produced electric energy was required to operate the biogas plant. The consumer unit agitators with 4.3% (in 2010) and 4.0% (in 2011) and the CHP unit with 2.5% (in 2010 and 2011) accounted for the highest electrical power demand, in relation to the electric energy produced by the CHP unit. Calculations show that the agitators consumed 51% (in 2010) and 46% (in 2011) of the total electric energy demand. The results stress the need for further research in the fields of substrate homogenization in biogas plants in order to reduce the demand for electric energy. Based on the results of electric energy consumption, follow-up studies have been conducted on nutrient distribution, which depends on agitator type and agitator regime. The investigation showed that significant differences in local concentrations of organic acids, which are not correlated to DM content, are found in dependence on agitator type and agitation regime. Measurements on electric energy consumption of the different agitator types verified that, depending on the agitator type, the saving potential rises up to 70%. The results for emissions and efficiency of the CHP unit confirm the fact that after readjustment of the air-fuel ratio (Lambda value), the emission values for NOx decline while CO increases. However, the emission-optimized operation mode leads to lower engine efficiency. The permanent measurements proved their legitimacy showing various emission deviations from the limiting values prior and post maintenance. In addition, the results show that by monitoring the lubricating oil quality, the oil change intervals can be maximized, while ensuring that engine performance is not endangered. This allows the operator to reduce maintenance expenditures while minimizing wear. To increase engine efficiency, the reduction of the lambda value combined with exhaust gas scrubbing and exhaust gas power generation is a promising approach. However, that would presuppose a permanent and almost total removal of H2S from the biogas. The fourth part of the study examined the technical and economic feasibility of a Fixed Bed Trickling Bioreactor (FBTB) for external biological desulfurization of biogas. In contrast to well-established biological methods to oxidize H2S, the FBTB allows removal of these from the biogas process, thus ensuring a constant low H2S concentration in the biogas. The FBTB showed H2S removal efficiencies (RE) of 98% at temperatures between 30-40°C. A major decline in RE in a range of 21-45% was observed when temperature in the FBTB dropped to a range of 5-25°C. The results revealed that different pH values of the percolation fluid and air ratios have little effect on RE. The practical use of the investigated FBTB system is an interesting technological alternative as disadvantages of internal biological desulfurization methods are being avoided. Due to high expenditures for operation resources and maintenance for FBTB operation during the research, a technical optimization is necessary to ensure economical operation. The results presented in this thesis show that the scientific instrument ?research biogas plant? is the ideal supplement to methods such as laboratory scale research and measuring programs. Research at full scale offers an entirely new opportunity to determine the interaction of process technique and process biology and to conduct long-term studies of gas utilization. Compared to measuring programs at commercial biogas plants, the research biogas plant has the advantage of being significantly better equipped with measurement technologies and that economic success is not the overall goal.
Acrocomia aculeata fruits from three regions in Costa Rica: An assessment of biometric parameters, oil content and oil fatty acid composition to evaluate industrial potential
(2020) Alfaro-Solís, Jose David; Montoya-Arroyo, Alexander; Jiménez, Víctor M.; Arnáez-Serrano, Elizabeth; Pérez, Jason; Vetter, Walter; Frank, Jan; Lewandowski, Iris
Due to increased global demand for vegetable oils, diversification of the supply chain with sustainable sources is necessary. Acrocomia aculeata has recently gained attention as a multi-purpose, sustainable crop for oil production. However, the information necessary for effective selection of promising varieties for agricultural production is lacking. The aim of this study was to assess variability in fruit morphology and oil composition of individual Acrocomia aculeata plants growing wild in different climatic regions of Costa Rica. Fruits at the same ripening stage were collected at three locations, and biometric features, oil content, fatty acid composition of oils from kernels and pulp, as well as fiber composition of husks were determined. Biometric parameters showed high variability among the regions assessed. Moreover, oil content and relative proportions of unsaturated fatty acids were higher at the most tropical location, whereas lauric acid content was lowest under these conditions, indicating a potential environmental effect on oil composition. Pulp oil content correlated positively with annual precipitation and relative humidity, but no clear relation to temperature was observed. The oil chemical composition was similar to that reported for Elaeis guineensis, suggesting that Acrocomia aculeata from Costa Rica may be a suitable alternative for industrial applications currently based on African palm oil. Analysis of husks as a coproduct revealed the possibility of obtaining materials with high lignin and low water and ash contents that could be used as a solid bioenergy source. In conclusion, Acrocomia aculeata oil is a promising alternative for industrial applications currently based on African palm oil and byproducts of its oil production could find additional use as a renewable energy source.
Advancing soybean adaptation to Central European growth conditions with novel breeding tools
(2020) Jähne, Felix; Würschum, Tobias
According to the European Soy Monitor 2018 (European Soy Monitor, 2018), there is a wide discrepancy in the EU between market demands and general sustainability aims regarding soybean products. Europe needs to take action, if it wants to maintain its protein demands and at the same time requests a reduction in the destruction of globally important tropical and subtropical ecosystems. One step towards more sustainable soybean products lies in the increase of domestic production which has the potential to decrease soybean imports from areas of unsustainable cultivation. An augmented EU production of soybeans can be achieved for example by increasing the yield potential of soybeans in areas where successful cultivation already takes place or by expanding the cultivation area to more northern parts of Central Europe. Breeding for new, improved and adapted soybean cultivars that meet those terms, is a key activity towards that aim. This dissertation elucidates three different ways how the adaptation of soybeans to the climatic and photoperiodic conditions of Central Europe can be assisted and even accelerated: 1) By using off-season climate-controlled LED chambers to enable a speed breeding single seed descent approach. A 10 h light regime, rich in blue and deprived of far-red light emission is capable to significantly reduce and synchronise the generation time of soybeans. It was possible to shorten the life cycle for a panel of 8 soybean cultivars from different maturity groups to 77 days. This allows several generations of soybeans to be grown within one year. For the short day crops rice and amaranth on the other hand, different light quality parameters were favoured. In those crops mean flowering time was accelerated when far-red light was included in the light protocol. This underlines the importance of a crop-specific light regime in order to realise the full potential of LED-based speed breeding single seed descent. 2) By including experiments in climate-control chambers in combination with molecular tools (i.e. genomic prediction) to advance cold tolerance in soybeans. This quantitatively inherited key trait is necessary to adapt soybeans to colder regions and consequently extend growing areas of this crop to higher latitudes in Europe. In the biparental soybean population Merlin × Sigalia (103 recombinant inbred lines) three QTL for cold tolerance during pod onset were found on chromosomes 7, 11 and 13. The relatively small proportion of genotypic variance for this trait explained by these QTL underlines the quantitative nature of cold tolerance. Genomic prediction was shown to be a promising approach to select for cold stress tolerance. Scenarios with different test set sizes and prediction models were evaluated. In scenarios with smaller test set sizes prediction accuracies increased if known and confirmed QTL were included in the prediction model. 3) By incorporating citizen science into the breeding process. The citizen science project ‘1000 Gärten’ from 2016 approached this topic. Phenotypic data from soybean cultivars and breeding lines were collected by citizen scientists in 2492 gardens throughout Germany which generated a unique dataset. Among many other results this study was able to show that in 2016 and within the early maturity segment of soybeans the factor temperature influenced flowering and maturity to a higher degree than photoperiod although day length differed by over an hour between the north and the south of Germany during the time of flowering. It was shown that this admittedly challenging tool can realise a significant impact not only regarding the possibility of a highly multi-environmental screening of breeding material but also by connecting plant breeding, agriculture and potential future costumers in order to raise awareness and acceptance of a crop in larger parts of the society - a factor that may not be highlighted enough when a new crop is introduced to our agriculture. These approaches should not be seen as an alternative to classical plant breeding, but rather considered as valuable additional tools that can contribute to conventional breeding of soybeans, as well as other crops. If applied, the presented tools may assist plant breeding to pave Europe’s way towards a greener and more sustainable future that is urgently needed.
Analyzing resistance to ergot caused by Claviceps purpurea [Fr.] Tul. and alkaloid contamination in winter rye (Secale cereale L.)
(2022) Kodisch, Anna; Miedaner, Thomas
Ergot caused by Claviceps purpurea [Fr.] Tul. is one of the oldest well-known plant diseases leading already in medieval times to severe epidemic outbreaks. After the occurrence of honeydew, the characteristic ergot bodies called sclerotia are formed on the ear. These are containing toxic ergot alkaloids (EAs). Strict limits are set within the European Union. Rye (Secale cereale L.) as cross-pollinating crop is particularly vulnerable to ergot since the competitive situation of fungal spores and pollen during flowering. Nevertheless, even today the threat is real as agricultural practice is changing and screening studies revealed EAs in samples of the whole cereal value chain frequently. The aims were to establish a harmonized method to test ergot resistance and EA contamination in winter rye, to clarify major significant factors and their relevance and to reveal the suitability of one commercial ELISA test. Further, effort was paid to examine the covariation of ergot amount and EA content considering different factors because of prospective legislative changes. Genotypes showed significant variation for ergot severity and pollen-fertility restoration after natural infection as well as artificial inoculation whereas a high positive correlation could be found between both treatments. Additionally, variances of environment, general combining ability (GCA), specific combining ability (SCA), and interactions were significant. Although male pollen-fertility restoration was of utmost importance, the female component was also significant. This illustrates that apart from promising selection of high restoration ability the maternal restorability could be exploited in future breeding programs especially when a high pollen amount is already reached. A large-scale calibration study was performed to clarify the covariation of ergot severity, EA content (HPLC, ELISA) considering genotypes, locations, countries, years, and isolates. EA profile was rather stable across country-specific isolates although large differences regarding the EA content were detected. The moderate covariation between ergot severity and EA content (HPLC) indicates that a reliable prediction of the EA content based on ergot severity is not possible what can also not be explained by grouping effects of the factors. Further, EAs seem not to act as virulence factor in the infection process since EA content showed no relationship to disease severity. Additionally, the missing correlation of ELISA and HPLC leads to the conclusion that the ELISA is not an appropriate tool what can be used safely to screen samples regarding ergot in the daily life. The genetic variation of male-sterile CMS-single crosses was analysed in a special design without pollen in field and greenhouse to identify resistance mechanisms and to clarify whether ergot can be reduced in the female flower. At this, comparison of needle and spray inoculation revealed medium to high correlations illustrating that both methods were suitable for this research. Significant environment and genotype by environment interaction variances were detected. So, testing across several environments is necessary also without pollen. Further, small but significant genotypic variation and identification of one more ergot-resilient candidate revealed that selection of female lines could be promising to further reduce ergot. The EA content was lower for less susceptible genotypes. Thus, EA content can be considerably reduced by breeding. A strong positive correlation could be found for ergot severity and EA content when analysing 15 factorial single crosses. The male pollen-fertility restoration was also here the most relevant component but the female component contributed an obviously higher proportion for the EA content than for ergot severity. In conclusion, this thesis demonstrate that implementing of a high and environmental stable male fertility restoration ability via exotic Rf genes can effectively reduce ergot although also the female restorability enables great opportunities. The unpredictable covariation between ergot amount and EA content illustrates that both traits have to be assessed, in particular the EA content by a valid HPLC approach to guarantee food and feed safety.
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2000
(2009) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2001
(2009) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2002
(2009) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2003
(2009) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2004
(2005) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2005
(2006) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2006
(2007) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2007
(2008) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2008
(2009) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2009
(2010) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2011
(2012) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2012
(2013) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2013
(2014) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2014
(2015) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2015
(2016) Landesanstalt für Bienenkunde; Rosenkranz, Peter
Bericht der Landesanstalt für Bienenkunde der Universität Hohenheim für das Jahr 2016
(2017) Landesanstalt für Bienenkunde; Rosenkranz, Peter

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